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Unstimulated samples were analyzed to verify the effect of the stimulation. Evaluation of cytokine production was based on percentage of positive cells, after subtracting the percentage of cells staining above the threshold for positivity in the negative control.Recovery ExperimentsRecovery experiments were performed in parallel on five different unstimulated RPMI 1640 diluted (1/5, vol:vol), IFN free, normal PB samples and their corresponding plasmas. For that purpose, precisely defined amounts of IFN (1250 and 5000 pg/ml) were added in parallel to each blood and plasma paired samples.
Hi I have a Fisher Paykel GWL10US model washer it is new to me (given to me second hand) and I just moved to a new home where I am using it for the first time. I can advance to the rinse cycle it will fill but beeps and will not agitate. On both the washing and rinsing settings, the washer fills with water past the high water mark and if I were not standin - [Read More] |
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